LOCUS       40924_4110       13100 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pAgc-A2-bgyg-3'N DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Ikeda Y, Ito K, Izumi Y, Shinomura T.
  TITLE     A candidate enhancer element responsible for high-level expression 
            of the aggrecan gene in chondrocytes
  JOURNAL   J. Biochem. 156 (1), 21-28 (2014)
  PUBMED    24554731
REFERENCE   2  (bases 1 to 13100)
  AUTHORS   Ikeda Y, Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and 
            Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, 
            Tokyo 113-8549, Japan URL :http://www.tmd.ac.jp/
REFERENCE   3  (bases 1 to 13100)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13100)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2014"; volume: "156"; issue: "1"; pages: "21-28"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and Dental 
            University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, Tokyo 
            113-8549, Japan URL :http://www.tmd.ac.jp/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      17..550
                     /gene="bgyg"
                     /label=rat Agc1 promoter sequence
                     /note="rat Agc1 promoter sequence"
                     /regulatory_class="promoter"
     exon            551..931
                     /gene="bgyg"
                     /note="rat Agc1 exon 1 sequence"
     intron          932..4705
                     /gene="bgyg"
     misc_binding    1560..1964
                     /gene="bgyg"
                     /label=SOX9 binding site
                     /bound_moiety="SOX9"
                     /note="A2"
     CDS             4887..7928
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             7944..8978
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     promoter        complement(9969..9987)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10005..10021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10029..10045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10053..10083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10098..10119)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10407..10995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11169..12026)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12027..12131)
                     /label=AmpR promoter
     rep_origin      12465..12920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     13061..13077
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcgaattc gagctcggta ccccaccccc aaaactttcc aacactgttc tgtaagggtt
       61 ctttcaagta gcattcccag ggtagggggt cgtcctgcgc gatcgcatct ttctgggtct
      121 cagctgcaaa tcgcctccag gtatgggggg ggaaccaccc ctccccgcgg ccagggcctg
      181 tcattctgac agcaggagtc gggtgctttc ggcactctgc gggcacagcc ctccggggcc
      241 tcctggactg cggagcttac gaccgcaatg cagacccggg tcctccagcg ctaccagcac
      301 agctttcctc cgcggcccca ggagctgcgg ggtccgcgct cctctcgtgc cctgcgcgcc
      361 cggcgccttc cccagtgagc gcgggtcccc agccctggtc cagagcgctc cggacgtctc
      421 tgccttcccc tccccctgcg aactggcccc gggggtgggg tttccctgtg cgctcgcccc
      481 cacccctagt ttgtgccctc ccctcccccg cccgccccta tgtatgtgtc accgcgcacc
      541 attcccgccc acctacctcc ccgcggtgcc agaggggctc acagagctga ggacgcgtgc
      601 agtaccgccc aaggtccctc attctccgcg actagcatct gacacatgtg ccgaaggaac
      661 tcaagacacc tttctgtatc ccttccctcc atcgaccctg ctccgaaacc cagccagcca
      721 gctaccactg cggccaccgc ccgaggggac ttgcggagga gacgccggcg ggaggagggc
      781 ttcgcagccc ccacccccac ccccactccc agcccagagc atccctgctg cagcgcagca
      841 agacccgggg ctgccagccc ccaggaatcc ctagctgctt agcagggata acggactgaa
      901 gttcttggag gagcgagttc aactcttcaa ggtaaagaaa gggctccttc tctccagagg
      961 agctctgtcc cagggcatct gggaatgctg ctgaggacgc gcgcctaagt gctgggggtg
     1021 gcgtgccttg gtgcccacat tgagtatgtg tgttggggac tcaggacatt agctgcatgc
     1081 ccatggaagt tgagcaaact caactgctgc cccgactcaa agtgaatggg aacacgtgga
     1141 ggggctcctg ctcagagtac acagaattaa gaaagaccag agctttttca agtttaaagt
     1201 accggggcgc taaactgaag tgtagagcga gaaacagttt taaagccaag gaaatgtgtg
     1261 ttcaaacccc actccccccc gcaacccctc gttcctctgg gcgctccata aggctttcat
     1321 attctcttgg gaaggccaac tgatcagaga atcagcaaaa ttatgtcgag ggggggcccg
     1381 gtacccgggg atcctcgggc ttcctgctgg gtcatcttcc atgcctctga gccccattgc
     1441 tgtccatctc aggcctccct cttcctctcc ccatccccag ggccctccat gtaccatccc
     1501 ttcccacggc ctcactttcc ttcccactga gaaaggtctc tctgtccacg agtgagcgtg
     1561 ggctggcaaa gtcggtcgcc tcacataaaa gcctcactga gtctctgaga ggcctggagc
     1621 ccagacagaa tggatttgct ccttattttc tttacactga gaaaacacac agagacacaa
     1681 gagcatattt attgcaataa ttctatcccg aagtttttaa gaagaagaaa taaaattagt
     1741 tttgctgccc actccacacc agacccctcc ctttcattcc ttcacatttc ctggtctaag
     1801 ccagggagaa aaacagagcc agcccaagcc ccacagagca ccattgcacc ccagacaatg
     1861 tccagcttgt tcagagagcg aggaagaaac acaactccga aacatacaca agacttccaa
     1921 gtgaagggtg ttctgaggga ggaacaggct agctttgtgt ctgagtcttt ggtaaagctc
     1981 cctctgcaaa cacacctcag gccgatcttc cctgaccctc caggaagccc tgactggttg
     2041 actgaacttg aacaggagcc cctctccaac tctgtgtctc ctcagagtac cagacatggg
     2101 gccccagaaa atattttaaa ttagtatcta aatcagaaaa atccccaacg agaagcaagg
     2161 ggccatttct tggaactatg gaactttcca gactttatac agcgtggggg tgatgtgcct
     2221 ctaccccctt agacatacag acctttcatt tctcagacat ggataaagga cggtggtcaa
     2281 aatggccaag ttccagagat aggaaagggt gactttgtcc tgcacccaga ctgttcctcc
     2341 ccagggactc cagaatgtca gtgtctcctc tccccacaaa cttgtgctcc taagggtgct
     2401 gctccctgag ctctgcgctg gtctagccca gcccatggtg agtaagcaga gagcgacacc
     2461 tgctggacca tttgactccg aaagctgagg ttgaggtgtg gaggctgggg ttctgtctcc
     2521 acgaaggatg ctctctgtgg ctgcagaccc catctgtccc ttactgttgc cccatcccca
     2581 aggcttcctt tcttcttctt ggaccaaaag taaatgctgc tgccgctgag cacaggggac
     2641 agggccacac agtacaagtc tcagaaggtg tgtgtgagac atggttgtgt aaccctccag
     2701 ggcagtggta ccacgaggtc cagactgttt ctacatgcag ctggctggat gctattgctc
     2761 cagatgatta cttgacaaat tcagttatgc ttgctgaatg tttggggaag agctttaagt
     2821 ggtggttgca acagatctgt gtacacaaat agcaactgac cagctgatga caatcaggga
     2881 agcttgactc taattcacgt ggccataaga aggccacagg agtcctaggt gctcacctcc
     2941 tggtatttaa ttgttcctct cacagcctga tcattgcagg gaaatggggg gggtggttgt
     3001 aaagagaggt ggatgtgggg gaataccctt acgggggagg gagagaggac agtgtgatta
     3061 tggacaggaa accgggaaag ggaataacat ttgaaatgta aataaagaaa tatatccaat
     3121 taaaaaaaaa aaagaaagag ctgtcttcct gattctagcc aggaccttga atcttaccta
     3181 aaactttgac acatgatcct tgctaaaata ttcgaggttc ctgaatttga agtgggtatg
     3241 tcatccccag attctgtcag ggaagacaga tcgtttgtca tttccgaagg gtcatgttag
     3301 ggttctttaa agaaacaatt ctctctccct tcttcccttt ttcctctcac ctccttccct
     3361 ctctctccct ccctctctct cctcccctct tcctccccat ctctttctcc accactcttt
     3421 attcctcctc ctccctcctc cctttcttct ctcgctccct ctcctatgtc attagtgggc
     3481 ttgagcatat cttaagtggc tcagtcactc agcagagctg ttgctgcgtg tgcacatgtt
     3541 cacatgtcca catgtgcacg tgtgagtcac tgggtatcaa gcatgttagg aaaacactcg
     3601 agcactgaac cacaccctaa ccttccacgt ggcttcttga tgcaattaag atgcaccata
     3661 aatgctaagg gtctggggct gcttagtgag tccctggtgg acggtctcaa tcacgtttct
     3721 gataagcagt gatgcacgct aagagtaaag acacagagta gagtacagga aatacactgt
     3781 aaccaccaat tattgtcact gccaagtctc ctgtactgta tacataatta cgttccccat
     3841 actttcatat gactggcacg cagtagattg gcgtgcaccg tcatctttgc agacaggtga
     3901 gaagtgtgtc gcactgcgac agtgcagtgt ctccaggcaa tggtgagttt ttcgcctcca
     3961 ctagaatctt cttgttctct gggtgctcca ccattgacca gtcatttctt ttcagcagag
     4021 gacatcccct tgcatggctc tgtgtgtctg tgtgtgtgtc acagggagag ggagagaatc
     4081 aagacaatac aaggggaggc tgtggttctc cagagctgca gagactccca ggaggccaag
     4141 gcagctggct gcacatactc agtttgaagc tccacatgtt caagccccag gaaaagctga
     4201 tgcctcaggt caagtccaaa gggaggagaa agtcagcggg aaggccatca gggaagaagt
     4261 ctctctccct ccacagaggg tccttttttg tttaaagcct tcatgggggt ggctgtgacc
     4321 cacccacact gaaaagggag gccattctag gtctacagac tccagagttt atgcaaaacc
     4381 cctcacagat atagaagaga gcataaacct gagcatctat ggtccgcctg tcacatatca
     4441 ctggctaccc caggaactca ttaggatgag tctccactgg ctaggagatg ggggaaccag
     4501 tacaccagcc atcttggaat taagctaaga aaggtcgaag gtttctgagg aaaggattct
     4561 tagtgaaaac actgtccaaa ccatgacaac ccgctgttgt gcaatgtgac ttcagagcct
     4621 tgggcatgaa ttttatcact tttttttctt cctttaacta tcccgtgctt taactgtcca
     4681 aataacatcc ctgttgtctc ttccagctga actctgtcga cggtatcgat aagcttggat
     4741 ccccagcttg ggggatcgaa agagcctgct aaagcaaaaa agaagtcacc atgtcgttta
     4801 ctttgaccaa caagaacgtg attttcgttg ccggtctggg aggcattggt ctggacacca
     4861 gcaaggagct gctcaagcgc gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     4921 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     4981 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
     5041 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
     5101 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
     5161 acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
     5221 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
     5281 gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg
     5341 gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg
     5401 gagaaaaccg cctcgcggtg atggtgctgc gttggagtga cggcagttat ctggaagatc
     5461 aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta
     5521 cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac
     5581 tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt
     5641 tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg
     5701 atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac
     5761 tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg
     5821 gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg
     5881 gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc
     5941 atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga
     6001 agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca
     6061 cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca
     6121 tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg
     6181 taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga
     6241 atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc
     6301 cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt
     6361 gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca
     6421 tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc
     6481 acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc
     6541 cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg
     6601 aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc
     6661 agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag
     6721 caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca
     6781 gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg
     6841 gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga
     6901 ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg
     6961 tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt
     7021 ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc
     7081 tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc
     7141 gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc
     7201 tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc
     7261 gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag
     7321 cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc
     7381 acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg
     7441 gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg
     7501 cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat
     7561 tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc
     7621 tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg
     7681 ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca
     7741 gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag
     7801 aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga
     7861 gcccgtcagt atcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct
     7921 ggtgtcaggg gatcctcgcc accatgaaaa agcctgaact caccgcgacg tctgtcgaga
     7981 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     8041 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     8101 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     8161 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     8221 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     8281 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     8341 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     8401 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     8461 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     8521 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     8581 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     8641 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     8701 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     8761 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     8821 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     8881 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     8941 ccagcactcg tccggatcgg gagatggggg aggctaactg acccgggact agtcgacctg
     9001 cagccaacac ctacaagcac aggctacaga agcggagcat gagacccaca cggaggagcc
     9061 gccccagcat ggcccactga gaggagcttc cacaatgtgc ccaggatgct gagcccagcg
     9121 gccagccatg ctgaccgtgc atcccactca catggtgtct tcttgtcgct ttttgtcata
     9181 taaggaatcc attaaagaag gaaaaaaaag ccacattgtg tgtgccccac tccccatatc
     9241 acccaaactg catctaattt atccaccacc atcaccgccc cccataaaaa accgccaaag
     9301 caaattcact gtaacccatg gactgagttt agagacattt ctttcatttc ccaccgtgcc
     9361 tttccaggga ccagtgcagg gacagggtga gaagagttaa attaaataaa gaagattatt
     9421 ttttgttgtt gcttcctgac tttacccaag aacagcacaa ttgttggtta cttcacctcc
     9481 agggagagct aggggccaga ggtggaattg gaaggagaca gggccaggga gagaggagta
     9541 gggcagccac acagaccaga gctttaagga ctcagaagag gcaaactata gcttagtgtg
     9601 ttggacaaaa gggatcgagg tctgtgccat ctatgtggga aggagcctgg ggcagggggc
     9661 aggctggcca aagggaagtg aagttgcctt tcctccaggg cccctctcct aggaaccttt
     9721 ctcctaggaa cctttcttct gggtcaacca ccctagcagg tgtcatttct cggctggact
     9781 gggtaggggc acttccttcc caggagtgct ccgccgtccc cttccctctc cccaccccgg
     9841 gacggtgcag gcaccagtgt tccgtgcacc tatttatatt tttgaaaact aaagattatg
     9901 ataattataa taataaagac attggaagag atctgctagc tcgagaattc gatatcaagc
     9961 ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct
    10021 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    10081 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    10141 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    10201 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    10261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10381 cgtaaaaagg ccgcgttgct ggcgtttttc gataggctcc gcccccctga cgagcatcac
    10441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    10861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    11041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    11101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    11161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    11221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    11281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    11341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11461 cgcaacgttg ttggcattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aataccgcgc ccggcgaccg
    11761 agttgctctt gcccggcgtc aatacgggat aatagtgtat gacatagcag aactttaaaa
    11821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    12001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    12061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    12121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    12181 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    12241 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    12301 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    12361 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    12421 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgacgcgc cctgtagcgg
    12481 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    12541 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    12601 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagagctt tacggcacct
    12661 cgaccgcaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
    12721 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    12781 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    12841 ttcggcctat tggttaaaaa atgagctgat ttaacaaata tttaacgcga attttaacaa
    12901 aatattaacg tttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    12961 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    13021 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
    13081 ttgtaatacg actcactata
//
